Biocatalysis by immobilized enzymes in a rotating bed reactor
Time lapse video showing how straightforward it is to use immobilized enzymes in a rotating bed reactor. A substrate giving a yellow coloured product was used to follow the reaction progress of an ester hydrolysis by an immobilized lipase. This substrate is commonly used to screen and characterize lipases.
Conditions: 4-nitrophenyl octanoate (30 mg) in aqueous phosphate buffer (150 mL, 0.1 M, pH 7.4, 0.1% Triton X100) was hydrolyzed at 30 °C using Purolite® CalB immoplus™ (150 mg) filled into a SpinChem® rotating bed reactor (RBR) S221 rotating at 500 rpm within a SpinChem® flower-baffled reaction vessel V211. The reaction was monitored for 30 min by filming the formation of the yellow 4-nitrophenoxide product (abs. max. ca 405 nm).