Screening of immobilized lipases using magnetic rotating bed reactors
Six different immobilized lipases were screened in parallel for the esterification of lauric acid into propyl laurate using the pre-packed SpinChem® MagRBR Lipase screening kit. The process proved fast and simple, as efficient sampling and monitoring of the process was achieved without filtration steps, by keeping the immobilized catalyst confined inside the MagRBR.
Conditions: Six MagRBRs, each packed with 0.5 mL of one of the immobilized enzymes, were spun in parallel on a six-position magnetic plate at 500 rpm for 1 h in preheated (60°C) substrate solution containing lauric acid (8.01 g, 40 mmol/vial), 1-propanol (2.4 g, 40 mmol/vial), and water (0.32 g, 18 mmol/vial). Samples were collected at 0, 10, 20, 30 and 60 min. Analysis of the propyl laurate product was done using GC-FID after 1:50 dilution in heptane containing internal standards (tetradecane). Formation of propyl laurate over time for esterification reactions catalysed by immobilized lipases CalB (lipase B from Candida antarctica), CalA (lipase A from Candida antarctica), TL (lipase from Thermomyces lanuginosa), RM (lipase from Rhizomucor miehei), CR (lipase from Candida rugosa) and PS (lipase from Pseudomonas cepacia).