Applications with keyword: Immobilized enzymes

Application 1028

The SpinChem® MagRBR ECR screening kit, pre-packed with Purolite® Lifetech™ resins, was used to screen six different enzyme carrier resins in parallel for the immobilization of lipase CalB. Easy sampling and monitoring of the process, together with effortless handling, established the MagRBR as a time and labour efficient screening device.

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    Conditions: The enzyme solution was prepared for each type of resin according to the protocols outlined in the SpinChem guidelines for enzyme immobilization (available at www.spinchem.com/support). Each MagRBR contained 0.5 mL of ECR carrier resin, and was spun in enzyme supernatant at 500 rpm for 24 h. To monitor the immobilization process, enzymatic activity in the supernatant was measured at five points during a 24 h timespan to determine the amount of residual enzyme in the solution. The enzymatic activity was determined using a lipase activity test based on the transformation of p-nitrophenylbutyrate to p-nitrophenol and butyric acid. The reaction was quantified by the use of a spectrometer through an increase in absorbance at 450 nm due to the conversion into the products.

Application 1027

Six different immobilized lipases were screened in parallel for the esterification of lauric acid into propyl laurate using the pre-packed SpinChem® MagRBR Lipase screening kit. The process proved fast and simple, as efficient sampling and monitoring of the process was achieved without filtration steps, by keeping the immobilized catalyst confined inside the MagRBR.

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    Conditions: Six MagRBRs, each packed with 0.5 mL of one of the immobilized enzymes, were spun in parallel on a six-position magnetic plate at 500 rpm for 1 h in preheated (60°C) substrate solution containing lauric acid (8.01 g, 40 mmol/vial), 1-propanol (2.4 g, 40 mmol/vial), and water (0.32 g, 18 mmol/vial). Samples were collected at 0, 10, 20, 30 and 60 min. Analysis of the propyl laurate product was done using GC-FID after 1:50 dilution in heptane containing internal standards (tetradecane). Formation of propyl laurate over time for esterification reactions catalysed by immobilized lipases CalB (lipase B from Candida antarctica), CalA (lipase A from Candida antarctica), TL (lipase from Thermomyces lanuginosa), RM (lipase from Rhizomucor miehei), CR (lipase from Candida rugosa) and PS (lipase from Pseudomonas cepacia).

Application 9002

The stable reaction environment in the EasyMax™ 102 Advanced synthesis workstation and the high flow rates through the SpinChem® RBR allowed for quick and convenient screening of different immobilized lipases to find the enzyme most suitable for further reaction optimization.

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    Conditions: A SpinChem® S2 RBR fitted with cartridges (4×2 mL) containing one enzyme at a time from Purolite® Lifetech™ lipase kit, was rotated at 500 rpm in a preheated (60 °C) substrate solution containing lauric acid (84.1 g), 1-propanol (31.5 mL) and water (3.36 mL). Analysis of propyl laurate product was accomplished by GC-FID after 1:50 dilution in heptane containing internal standard (tetradecane). Reactions were repeated twice.

Application 9001

Immobilized catalyst recycling using a SpinChem® rotating bed reactor (RBR) and a Mettler-Toledo EasyMax™ 102 Advanced synthesis workstation. The process proved very time efficient as no filtration steps were needed between cycles, or for the samples extracted for analysis during each run. Washing of the resin between runs was fast, simple and robust, without running the risk of material loss.

Products: SpinChem® RBR S2
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    Conditions: A SpinChem® S2 RBR containing Novozyme 435 (1 g), was rotated at 500 rpm in a preheated (30 °C) substrate solution containing 2-Ethyl-1-hexanol (5.21 g), Vinyl acetate (3.44 mL) and heptane (110 mL). The reaction was repeated for seven cycles, and samples for each run were taken after 0, 10, 20, 30 and 60 min. Analysis of 2-Ethylhexyl acetate product was done suing GC-FID after 1:9 dilution in heptane containing internal standard (tetradecane, 50 mmol). Between cycles, the resin was washed by spinning the RBR in heptane for 1 min.

Application 1023

Poster describing the screening of resins with immobilized enzymes for esterification reactions. The screening was performed with prepacked cartridges inside a SpinChem® rotating bed reactor (RBR) and compared to parallel screening with prototype disposable magnetic RBR (MagRBR) in 10 mL volume. Both approaches were very quick, required minimum preparation and no filtration. Up-scaled processes enabled quick enzyme recycling, opening for the possibility of semi-continuous processes with attractive production economy.

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    Conditions: This poster contains data from several reactions with immobilized enzymes performed at different conditions. The details of each reaction are displayed under each figure in the poster. This poster was presented at BioTrans2017 - the 13th international symposium on biocatalysis and biotransformations, in Budapest, Hungary, the 9th to 13th of July, 2017.

Application 1019

Poster on study of catalyst recycling during esterification and transesterification reactions with immobilized lipases in rotating bed reactors. Data from several laboratories showed that no attrition or grinding occurred and that no filtration was necessary between reaction cycles. The production capacity was estimated to 50 kilograms per gram of catalyst thanks to the high catalyst stability.

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    Conditions: This study comprised several different laboratories, immobilized enzymes, and conditions for performing esterification and transesterification reactions using a SpinChem® rotating bed reactor (RBR). The details of every reaction are displayed in connection to each figure on the poster. This poster was displayed at Biocat2016 - the 8th international congress on biocatalysis, at Hamburg University of Technology, Germany, August 28th to September 1st, 2016.

Application 1014

Time lapse video showing how straightforward it is to use immobilized enzymes in a rotating bed reactor. A substrate giving a yellow coloured product was used to follow the reaction progress of an ester hydrolysis by an immobilized lipase. This substrate is commonly used to screen and characterize lipases.

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    Conditions: 4-nitrophenyl octanoate (30 mg) in aqueous phosphate buffer (150 mL, 0.1 M, pH 7.4, 0.1% Triton X100) was hydrolysed at 30 °C using Purolite® CalB immoplus™ (150 mg) filled into a SpinChem® rotating bed reactor (RBR) S221 rotating at 500 rpm within a SpinChem® flower-baffled reaction vessel V211. The reaction was monitored for 30 min by filming the formation of the yellow 4-nitrophenoxide product (abs. max. ca 405 nm).

Application 1001

Investigation of how rotational speed influences the efficiency of rotating bed reactors (RBR) for a diversity of processes such as adsorption, neutralization and ammonolysis. It was demonstrated how reaction rates could reach a plateau with the SpinChem® RBR when mass transfer efficiency exceeded reaction speed.

Application brochure

Brochure with SpinChem® rotating bed reactors (RBR) – applications, products and technology. Learn from examples how to increase speed and convenience for heterogeneous reactions in laboratory development and production scale. Read about the capabilities and handling benefits with pre-packed cartridges.

Application brochure

Learn how SpinChem rotating bed reactors (RBR) can eliminate poor mass transfer in heterogeneous reactions during chemical synthesis and biotransformations. Preserve activity and facilitate recycling of solid phases with the RBR. This brochure shows technology and applications.

Application L1705

A comprehensive review of biocatalysis covering how the recent advances in engineering of enzymes, substrates, media and reactor design contributes to a sustainable chemistry development. The authors wrote that SpinChem® RBR technology “combines the advantages of a stirred tank with those of a packed bed” thus giving benefits by “circumventing mechanical attrition of the biocatalyst” while “mass transfer is greatly accelerated”.

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    Roger A. Sheldon, John M. Woodley
    Chemical Reviews, 117 (2017) in press

Application L1704

A tutoral review taking a holistic approach to the engineering of biotransformations utilising isolated enzymes. The authors highlighted SpinChem® RBR technology benefits and wrote; “… in addition to protecting the biocatalyst from shear forces caused by the stirrer, it greatly accelerates mass transfer, thus affording substantially higher reaction rates and creating the possibility to use much smaller reactors”.

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    Roger A. Sheldon, Pedro C. Pereira
    Chemical Society Reviews, 46 (2017) 2678-2691

Application L1703

A traditional stirred tank reactor setup was compared to a rotating bed reactor (RBR) for the biocatalytic synthesis of the anti-inflammatory drug (S)-naproxen. Both setups performed well during five repetitive bathes giving an enantiomeric excess of 99% and an isolated yield of 92%, but the RBR was easier to handle and the authors concluded that “… the rotating bed reactor concept can be regarded as a promising option for industrial applications”.

Products: SpinChem® RBR S2
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    M. Aßmann, A. Stöbener, C. Mügge, S. K. Gaßmeyer, L. Hilterhaus, R. Kourist, A. Liese, S. Kara
    Reaction Chemistry & Engineering, 2 (2017) 531-540

Application L1701

Katarzyna Szymańska, Klaudia Odrozek, Aurelia Zniszczoł, Wojciech Pudło, Andrzej B. Jarzębski
Chemical Engineering Journal, 315 (2017) 18-24

Products: SpinChem® RBR S2
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    Katarzyna Szymańska, Klaudia Odrozek, Aurelia Zniszczoł, Wojciech Pudło, Andrzej B. Jarzębski
    Chemical Engineering Journal, 315 (2017) 18-24

Application L1402

A synthesis of N-Boc (2R)-1,4-oxazepane-2-carboxylic acid was developed in 39% yield over seven steps starting from methyl (2R)-glycidate. The key step was a lipase-catalyzed regioselective lactamization performed using SpinChem® rotating bed reactor that simplified work up and recycling of the enzyme.

Products: SpinChem® RBR S3
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    Carl-Johan Aurell, Staffan Karlsson, Fritiof Pontén, Søren M. Andersen
    Organic Process Research & Development, 18 (2014) 1116-1119

Application L1301

Hendrik Mallin, Jan Muschiol, Dr. Emil Byström, Prof. Dr. Uwe T. Bornscheuer
ChemCatChem, 5 (2013) 3529-3532

Products: SpinChem® RBR S3
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    Hendrik Mallin, Jan Muschiol, Dr. Emil Byström, Prof. Dr. Uwe T. Bornscheuer
    ChemCatChem, 5 (2013) 3529-3532