Applications with keyword: Mettler-Toledo
This application note demonstrates how the combination of a SpinChem MagRBR custom-filled with heterogenous Pd(II)-catalyst and the controlled conditions in a Mettler-Toledo EasyMax™ 102 Advanced Synthesis Workstation allows for quick and convenient generation of C-C coupled products.
Blue dye was removed from a highly viscous liquid using a SpinChem® RBR S2 in an EasyMax™ 102 Advanced Synthesis Workstation. Monitoring of the reaction was easily recorded as no freely suspended ion exchange resin beads or resin debris interfered with the readings. This demonstrates that the RBR technology is extremely well suited for in-line monitoring. The viscosity of the solution was determined to ca 230 cP at 25°C, showing that it is possible to absorb dye even from a highly viscous solution.
The stable reaction environment in the EasyMax™ 102 Advanced synthesis workstation and the high flow rates through the SpinChem® RBR allowed for quick and convenient screening of different immobilized lipases to find the enzyme most suitable for further reaction optimization.
Conditions: A SpinChem® S2 RBR fitted with cartridges (4×2 mL) containing one enzyme at a time from Purolite® Lifetech™ lipase kit, was rotated at 500 rpm in a preheated (60 °C) substrate solution containing lauric acid (84.1 g), 1-propanol (31.5 mL) and water (3.36 mL). Analysis of propyl laurate product was accomplished by GC-FID after 1:50 dilution in heptane containing internal standard (tetradecane). Reactions were repeated twice.
Immobilized catalyst recycling using a SpinChem® rotating bed reactor (RBR) and a Mettler-Toledo EasyMax™ 102 Advanced synthesis workstation. The process proved very time efficient as no filtration steps were needed between cycles, or for the samples extracted for analysis during each run. Washing of the resin between runs was fast, simple and robust, without running the risk of material loss.
Conditions: A SpinChem® S2 RBR containing Novozyme 435 (1 g), was rotated at 500 rpm in a preheated (30 °C) substrate solution containing 2-Ethyl-1-hexanol (5.21 g), Vinyl acetate (3.44 mL) and heptane (110 mL). The reaction was repeated for seven cycles, and samples for each run were taken after 0, 10, 20, 30 and 60 min. Analysis of 2-Ethylhexyl acetate product was done suing GC-FID after 1:9 dilution in heptane containing internal standard (tetradecane, 50 mmol). Between cycles, the resin was washed by spinning the RBR in heptane for 1 min.