Applications with keyword: One-pot multistep

Application 9003

Two dyes were selectively extracted onto different adsorbents within the same run using a SpinChem® rotating bed reactor (RBR) and an EasyMax™ 102 Advanced synthesis workstation. This experiment illustrates performing cascade reaction for one-pot multi-step synthesis.

Products: SpinChem® RBR S2
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    Conditions: Allura red (60 μM) and methylene blue (31 μM) in deionized water (about 120 mL) were adsorbed onto Purolite® A500Plus (13 mL, 300-1200 μm) and Purosorb™ PAD700 (13 mL, 350-1200 μm), respectively. Each adsorbent was filled into two of the four compartments in a SpinChem® S2 rotating bed reactor (RBR) operated at 500 rpm within an EasyMax™ 102 Advanced synthesis workstation.

Application 1025

Video showing the formation of alginate beads under conditions mimicking whole cell encapsulation. The use of a SpinChem® rotating bed reactor (RBR) allowed easy collection, maturing and washing of the alginate beads. With the RBR setup, it was possible to immediately continue with filling of the reaction substrate into the same vessel, thus reducing the number of handling steps and facilitating bead recycling. The beads showed no signs of physical wear after use in the RBR.

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    Conditions: A SpinChem® S2 RBR with only outer filter was rotated at 50 rpm in an aqueous calcium chloride solution (200 mL, 50 mM) in a SpinChem® V2 flower-baffled reaction vessel, while an alginate solution (3.6%) mixed 1:1 with a simulated cell suspension consisting of 50 mM phosphate-buffered saline (PBS) coloured with methylene blue (100 mg/L), was added dropwise through a fine-tipped needle. The alginate beads formed instantaneously and were slowly drawn into the RBR where they were solidified for 10 min, after which the rotational speed was increased to 300 rpm and the vessel was drained from solution, washed with fresh aqueous calcium chloride solution and drained again. Pure water was added to mimic a substrate solution and finally the RBR was stopped and emptied from alginate beads which was observed to have an intact round morphology.

Application 1009

Video illustrating how a mixture of red and blue dyes with different chemical properties can be selectively extracted onto different adsorbents within the same run using a rotating bed reactor (RBR). The dyes were separated based on ionic and hydrophobic interactions, respectively.

Products: SpinChem® RBR S2
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    Conditions: Allura red (40 µM) and methylene blue (13 µM) in deionized water (about 160 mL) were adsorbed onto Amberlite IRA900 Cl (13 mL, 650-820 µm) and Amberlite XAD1600N (13 mL, 400±50 µm), respectively. Each adsorbent was filled into two of the four compartments in a SpinChem® S221 rotating bed reactor (RBR) operated at 800 rpm within a SpinChem® V221 flower-baffled reaction vessel. The total extraction time for one run was nine minutes and the video contains photos of the adsorbents before and after two repeated extractions.

Application brochure

Brochure with SpinChem® rotating bed reactors (RBR) – applications, products and technology. Learn from examples how to increase speed and convenience for heterogeneous reactions in laboratory development and production scale. Read about the capabilities and handling benefits with pre-packed cartridges.

Application L1706

This scientific minireview highlights that “one-pot multi-catalysis reactions is a revolutionary tool for multistep synthesis” and that “reaction engineering is indispensable to further advance biotransformation processes”. The authors concluded that “advanced reactor design such as … the SpinChem reactor, could also enhance cascade biotransformation processes”.

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    Shuke Wu, Zhi Li
    ChemCatChem, 9 (2017) in press