Applications with keyword: Quick recycling
Immobilized catalyst recycling using a SpinChem® rotating bed reactor (RBR) and a Mettler-Toledo EasyMax™ 102 Advanced synthesis workstation. The process proved very time efficient as no filtration steps were needed between cycles, or for the samples extracted for analysis during each run. Washing of the resin between runs was fast, simple and robust, without running the risk of material loss.
Conditions: A SpinChem® S2 RBR containing Novozyme 435 (1 g), was rotated at 500 rpm in a preheated (30 °C) substrate solution containing 2-Ethyl-1-hexanol (5.21 g), Vinyl acetate (3.44 mL) and heptane (110 mL). The reaction was repeated for seven cycles, and samples for each run were taken after 0, 10, 20, 30 and 60 min. Analysis of 2-Ethylhexyl acetate product was done suing GC-FID after 1:9 dilution in heptane containing internal standard (tetradecane, 50 mmol). Between cycles, the resin was washed by spinning the RBR in heptane for 1 min.
Poster describing the screening of resins with immobilized enzymes for esterification reactions. The screening was performed with prepacked cartridges inside a SpinChem® rotating bed reactor (RBR) and compared to parallel screening with prototype disposable magnetic RBR (MagRBR) in 10 mL volume. Both approaches were very quick, required minimum preparation and no filtration. Up-scaled processes enabled quick enzyme recycling, opening for the possibility of semi-continuous processes with attractive production economy.
Conditions: This poster contains data from several reactions with immobilized enzymes performed at different conditions. The details of each reaction are displayed under each figure in the poster. This poster was presented at BioTrans2017 - the 13th international symposium on biocatalysis and biotransformations, in Budapest, Hungary, the 9th to 13th of July, 2017.
Convenient transfer hydrogenation catalysed by palladium-containing beads was performed using a SpinChem® rotating bed reactor (RBR). The set-up resulted in high product conversions throughout more than 10 consecutive batches without any need for filtration to recycle the catalyst.
Poster on study of catalyst recycling during esterification and transesterification reactions with immobilized lipases in rotating bed reactors. Data from several laboratories showed that no attrition or grinding occurred and that no filtration was necessary between reaction cycles. The production capacity was estimated to 50 kilograms per gram of catalyst thanks to the high catalyst stability.
Conditions: This study comprised several different laboratories, immobilized enzymes, and conditions for performing esterification and transesterification reactions using a SpinChem® rotating bed reactor (RBR). The details of every reaction are displayed in connection to each figure on the poster. This poster was displayed at Biocat2016 - the 8th international congress on biocatalysis, at Hamburg University of Technology, Germany, August 28th to September 1st, 2016.
Comparison of SpinChem® rotating bed reactor (RBR) with traditional reaction set-ups for a demanding biotransformation. SpinChem® RBR matched or outperformed the other systems and gave a 10 to 25-fold more time-efficient recycling of the encapsulated cells.
Brochure with SpinChem® rotating bed reactors (RBR) – applications, products and technology. Learn from examples how to increase speed and convenience for heterogeneous reactions in laboratory development and production scale. Read about the capabilities and handling benefits with pre-packed cartridges.
A synthesis of N-Boc (2R)-1,4-oxazepane-2-carboxylic acid was developed in 39% yield over seven steps starting from methyl (2R)-glycidate. The key step was a lipase-catalyzed regioselective lactamization performed using SpinChem® rotating bed reactor that simplified work up and recycling of the enzyme.
Carl-Johan Aurell, Staffan Karlsson, Fritiof Pontén, Søren M. Andersen
Organic Process Research & Development, 18 (2014) 1116-1119