Whole cell biocatalysis is powerful, but not straightforward. One way of utilizing whole cells is to encapsulate them in a matrix such as alginate to make them easier to separate from a reaction mixture. However, alginate beads are not mechanically stable enough to be packed into columns and are easily destroyed in stirred tank reactors (STR). This makes enzyme recycling ineffective, at the same time as mass transfer limitations may prevail.
In this application note, the recycling of three types of enzymes immobilized in different ways was studied:
The breakdown of material was prevented by the use of a rotating bed reactor (RBR), protecting the beads from any mechanical damage. The mixing was enhanced and the mass transfer was greatly improved.
Recycling studies revealed that, for the transaminase reaction, the SpinChem RBR was superior:
To ensure a properly gas-saturated reaction mixture, we recommend using a Gas Sparger.
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For additional reading
Efficient biocatalysis with immobilized enzymes or encapsulated whole cell microorganism by using the SpinChem reactor system
Industries Pharmaceuticals and Cosmetics
Topics Biocatalysis, Process Development, Downstream Processing
Products SpinChem® RBR S3
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