Production of recombinant choline oxidase and its application in betaine production

The paper describes the author’s successful work to express and use recombinant choline oxidase.

The gene for the choline oxidase was isolated from a Gram-positive soil bacterium, cloned into an expression vector, inserted into and overexpressed in a Rosetta expression system.

The isolated recombinant choline oxidase was subsequently immobilized onto Ni-Sepharose beads, which were loaded in a rotating bed reactor (RBR).

With the immobilized choline oxidase in the RBR, reaction conditions such as pH and temperature were optimized and the enzymatic activity measured for the reaction of choline to glycine betaine via betaine aldehyde.