Peroxygenase-Driven Ethylbenzene Hydroxylation in a Rotating Bed Reactor

“The ability of unspecific peroxygenase (UPO) to hydroxylate a wide range of substrates with just H2O2 as a cosubstrate has attracted a great deal of attention in biocatalytic research. The enzyme’s intrinsic limitation to be inactivated by excess amounts of the oxidative cosubstrate has been tackled with in or ex situ hydrogen peroxide (H2O2) provision strategies. In this paper, we present the application of the covalently immobilized UPO mutant PaDa-I in a rotating bed reactor for the hydroxylation of ethylbenzene in a two-liquid-phase system. By monitoring product formation in the organic phase and H2O2 concentration in the aqueous phase, the multiphasic reaction was optimized.”

Highlights:

• Biphasic liquid
• Immobilized unspecific peroxygenase