Application L1701
A novel hierarchically structured siliceous packing to boost the performance of rotating bed enzymatic reactors

Katarzyna Szymańska, Klaudia Odrozek, Aurelia Zniszczoł, Wojciech Pudło, Andrzej B. JarzębskiChemical Engineering Journal, 315 (2017) 18-24 Keywords: Biotransformatio, Immobilized enzymes, Organic molecules, Scientific literature

Application L1801
Advanced oxidation process for the removal of ibuprofen from aqueous solution: A non-catalytic and catalytic ozonation study in a semi-batch reactor

Degradation of the pharmaceutical compound ibuprofen by ozone, was optimized in batch type reactors with and without zeolite based heterogeneous catalysts. The rotating bed reactor technology increased the concentrations of dissolved gas compared to traditional stirred tank reactors and allowed convenient handling of the catalyst particles without any filtration.  Keywords: Fast reaction, Gas-distribution, Organic molecules, Scientific literature, Zeolite

Application L1704
Biocatalysis engineering: the big picture

A tutoral review taking a holistic approach to the engineering of biotransformations utilising isolated enzymes. The authors highlighted SpinChem® RBR technology benefits and wrote; “… in addition to protecting the biocatalyst from shear forces caused by the stirrer, it greatly accelerates mass transfer, thus affording substantially higher reaction rates and creating the possibility to use much smaller reactors”.   Keywords: Biotransformation, Immobilized enzymes, Organic molecules, Review, Scientific literature

Application L1604
Catalytic hydrogenation of d-xylose over Ru decorated carbon foam catalyst in a SpinChem® rotating bed reactor

Tung Ngoc Pham, Ajaikumar Samikannu, Anne-Riikka Rautio, Koppany L. Juhasz, Zoltan Konya, Johan Wärnå, Krisztian Kordas, Jyri-Pekka MikkolaTopics in Catalysis, 59 (2016) 1165-1177

Application L1702
CFD modeling of flow and ion exchange kinetics in a rotating bed reactor system

A rotating bed reactor containing ion exchange beads was modeled in flower-baffled reaction vessels. It was shown that the baffles are vital for reducing surface vortexes and circular flow within the vessel. The authors concluded that the flow rates through the packed bed and reaction rates tend to increase with deeper baffles.   Keywords: Ion exchange, Scientific literature, Simulation, Technology

Application L1601
Deconstruction of Nordic hardwood in switchable ionic liquids and acylation of the dissolved cellulose

Valerie Eta, Jyri-Pekka MikkolaCarbohydrate Polymers, 136 (2016) 459-465

Application L1301
Efficient biocatalysis with immobilized enzymes or encapsulated whole cell microorganism by using the SpinChem reactor system

Hendrik Mallin, Jan Muschiol, Dr. Emil Byström, Prof. Dr. Uwe T. BornscheuerChemCatChem, 5 (2013) 3529-3532   "...the immobilized transaminase was better protected from mechanical forces in the SpinChem device."    Keywords: Alginate, Biotransformation, Encapsulated cells, Immobilized enzymes, Organic molecules, Scientific literature

Application L1401
Enhanced mass transfer upon switchable ionic liquid mediated wood fractionation

Valerie Eta, Ikenna Anugwom, Pasi Virtanen, P. Mäki-Arvelaa, Jyri-Pekka MikkolaIndustrial Crops and Products, 55 (2014) 109-115  Keywords: Scientific literature

Application L1802
Epoxidation of fatty acids and vegetable oils assisted by microwaves catalyzed by a cation exchange resin

Epoxidation reactions with in-situ formed percarboxylic acids were enhanced by heterogeneous catalysis and optimized with respect to product yield. The authors concluded that “SpinChem RBR, was beneficial, in terms of eliminating mass transfer limitations, it enabled a simpler collection and recycling of the catalyst and minimized mechanical wear of the solid catalyst”.  Keywords: Easy handling, Ion exchange, Organic molecules, Scientific literature, Synthesis

Application L1605
Fibre stress-strain response of high-temperature chemi-thermomechanical pulp treated with switchable ionic liquids

Ran Duan, Bo S. Westerlind, Magnus Norgren, Ikenna Anugwom, Pasi Virtanen, Jyri-Pekka MikkolaBioResources, 11 (2016) 8570-8588

Application L1603
In quest for the optimal delignification of lignocellulosic biomass using hydrated, SO2 switched DBU MEASIL switchable ionic liquid

Ikenna Anugwoma, Luis Rujana, Johan Wärnå, Mattias Hedenström, Jyri-Pekka MikkolaChemical Engineering Journal, 297 (2016) 256–264

Application L1402
Lipase catalyzed regioselective lactamization as a key step in the synthesis of N-Boc (2R)-1,4-oxazepane-2-carboxylic acid

A synthesis of N-Boc (2R)-1,4-oxazepane-2-carboxylic acid was developed in 39% yield over seven steps starting from methyl (2R)-glycidate. The key step was a lipase-catalyzed regioselective lactamization performed using SpinChem® rotating bed reactor that simplified work up and recycling of the enzyme. Carl-Johan Aurell*, Staffan Karlsson, Fritiof Pontén, and Søren M. Andersen Keywords: Biotransformation, Immobilized enzymes, Organic molecules, Quick recycling, Scientific literature

Application L1602
Modularized biocatalysis: Immobilization of whole cells for preparative applications in microaqueous organic solvents

Jochen Wachtmeister, Philip Mennicken, Andreas Hunold, Dörte RotherChemCatChem, 8 (2016) 607-614   Keywords: Biotransformation, Encapsulated cells, Organic molecules, Scientific literature

Application L1901
Progress on the pre-treatment of lignocellulosic biomass employing ionic liquids

Pobitra Halder, Sazal Kundu, Savankumar Patel, Adi Setiawan, Rob Atkin, Rajarathinam Parthasarthy, Jorge Paz-Ferreiro, Aravind Surapaneni, Kalpit ShahRenewable and Sustainable Energy Reviews, 105 (2019) 268-292 The paper refers to SpinChem Application L1603, with this quote "The model was analysed and optimised using Modest™ software and it was found that the ® SpinChem rotating bed reactor was the most promising reactor in term of lignin dissolution when compared to the non-stirring batch reactor and the loop reactor."  Keywords: Scientific literature

Application L1703
Reaction engineering of biocatalytic (S)-naproxen synthesis integrating in-line process monitoring by Raman spectroscopy

A traditional stirred tank reactor setup was compared to a rotating bed reactor (RBR) for the biocatalytic synthesis of the anti-inflammatory drug (S)-naproxen. Both setups performed well during five repetitive bathes giving an enantiomeric excess of 99% and an isolated yield of 92%, but the RBR was easier to handle and the authors concluded that “… the rotating bed reactor concept can be regarded as a promising option for industrial applications”. Keywords: Biotransformation, Immobilized enzymes, Organic molecules, Scientific literature

Application L1606
Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

Jochen Wachtmeister, Dörte RotherCurrent Opinion in Biotechnology, 42 (2016) 169-177 Keywords: Biotransformation, Encapsulated cells, Organic molecules, Scientific literature

Application L1705
Role of biocatalysis in sustainable chemistry

A comprehensive review of biocatalysis covering how the recent advances in engineering of enzymes, substrates, media and reactor design contributes to a sustainable chemistry development. The authors wrote that SpinChem® RBR technology “combines the advantages of a stirred tank with those of a packed bed” thus giving benefits by “circumventing mechanical attrition of the biocatalyst” while “mass transfer is greatly accelerated”.   Keywords: Biotransformation, Immobilized enzymes, Organic molecules, Review, Scientific literature

Application L2001
Using Spinchem Rotating Bed Reactor Technology for Immobilized Enzymatic Reactions: A Case Study

Subhash Pithani, Staffan Karlsson, Hans Emtenäs, and Christopher T. ÖbergOrganic Process Research & Development 2019 23 (9), 1926-1931 "By using SpiChem RBR technology, we found that recycling of the immobilized enzyme was easy with preserved enantioselectivity and catalytic activity. The final optimized process was successfully demonstrated on a 1 kg scale with 39% isolated yield and 98.8% enantiomeric purity."     Keywords: Scientific literature

Application L1706
Whole-cell cascade biotransformations for one-pot multistep organic synthesis

This scientific minireview highlights that “one-pot multi-catalysis reactions is a revolutionary tool for multistep synthesis” and that “reaction engineering is indispensable to further advance biotransformation processes”. The authors concluded that “advanced reactor design such as … the SpinChem reactor, could also enhance cascade biotransformation processes”.   Keywords: Biotransformation, Encapsulated cells, One-pot multistep, Review, Scientific literature

Application L2110
Process design of a continuous biotransformation with in situ product removal by cloud point extraction

In biotransformations, obstacles commonly encountered are product inhibition, product toxicity, and reaction equilibria that prevents complete conversion. Enzyme engineering has made tremendous progress in alleviating these problems. The concept of in situ product removal (ISPR) may still be an attractive alternative or complement. The authors have demonstrated concurrent enzymatic reaction and ISPR, referred to as 'extractive biocatalysis'. For the ISPR, the authors evaluated the use of aqueous micellar two-phase systems (ATPMS) as an extraction medium. For the model reaction, Penicillin G hydrolysis by CalB lipase, the demonstrated process was thus a continuous, heterogeneous extractive biocatalysis with cloud point extraction. An RBR was used during the process development work to determine the Michaelis-Menten kinetics of the CalB immobilized in gel coatings on column packing material. Also, the particles were easily re-used in stability experiments.

Application L2117
Multi‐enzyme cascade reaction in a miniplant two‐phase‐system : Model validation and mathematical optimization

In the American Institute of Chemical Engineers, AIChE Journal, the authors of this paper highlights the use of Rotating Bed Reactor (RBR) with two different immobilized enzymes at the same time in a cascade reaction. In the flow chart above of the miniplant consisting of a continuously stirred tank reactor (CSTR) equipped with an RBR (highlighted in orange) (a), a buffer tank (b), an extractive centrifuge (c) and a fixed bed reactor (d)  In the reaction scheme the complete multi-enzyme cascade is shown with the two enzymes placed in the RBR is highlighted. If you would like to get in contact with us give us a call or fill in the form.

Application L2005
Solvent and Rotating Bed Reactor Extraction with One- and Two-Phase Solvents Applied to Bilberries (Vaccinium myrtillus) for Isolating Valuable Antioxidants

Correlation data between extracted anthocyanidins/ anthocyanins vs time, temperature, and ethanol concentration was collected and analyzed. RBR extraction was deemed advantageous, in the authors’ own words: “The RBR was better than traditional extraction and 16 min sufficed.” Bilberry press cake is the major by-product from the production of bilberry juice. To valorize the press cake, the authors describe their work to extract antioxidant antocyanidins/ antocyanins from the cake. Different solvent compositions were compared as well as traditional extraction vs extraction by use of RBR. The methods used were as follows: “To summarize, the compared extraction methods were as follows: 1. Traditional extraction using different concentrations of ethanol dissolved in water, different masses of material, and different temperatures. 2. RBR extraction using different concentrations of ethanol dissolved in water, different masses, and temperatures. 3. RBR extraction using two-phase system, method 3 only mass and temperature were changed. 4. RBR extraction using two-phase system method 4, only mass and temperature were changed.”  

Application L2112
Production of hydroxytyrosol rich extract from Olea europaea leaf with enhanced biological activity using immobilized enzyme reactors

The paper describes work to valorize olive leaves, the major by-product in olive oil production. Aqueous olive leaf extract is rich in oleuropein that was enzymatically hydrolyzed by beta-glucosidase to hydroxytyrosol and other products. Compared to the non-modified extract, the modified one demonstrated 20% higher antioxidant activity, seven-fold higher antibacterial activity, and enhanced cytotoxicity against leiomyosarcoma cells. The beta-glucoside was immobilized in chitosan-coated magnetic beads and used in both stirred tank reactor (STR) and rotating bed reactor (RBR). The immobilized enzyme showed good stability over time and the activity was good in both STR and RBR settings. However, the enzyme beads were damaged mechanically in the STR and could not be re-used. The RBR solved the problem of bead attrition allowing the immobilized enzyme to be re-used and thus giving superior total turnover number to the STR.

Application L2119
Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors

This article involves the use of a rotating bed reactor for a biocatalytic process involving biofilms. The biofilm of Pseudomonas taiwanensis, with a genetically introduced Baeyer-Villiger monooxygenase (BVMO), was grown directly on 3D-printed polyamide inserts installed in the RBR S3. The biofilm, immobilized on the cartridges, was then used to catalyze the conversion of cyclohexanone. The authors comment that the work opens up the possibility to use biofilms in rotating bed reactors for synthesis via biocatalysis.

L-Asparaginase production in rotating bed reactor from Rhizopus microsporus IBBL-2 using immobilized Ca-alginate beads

The present work reports on the production of extracellular l-asparaginase from Rhizopus microsporus IBBL-2 using submerged fermentation (SmF) process free of glutaminase and urease activities. Scale-up studies involving 200-mL and 1-L rotating bed reactor (RBR) using immobilized beads were done and the results obtained are 20.21 U mL-1 and 19.13 U mL-1, respectively, the increased activity with immobilization accounts for reduced shear on cells due to increased stability as compared to the free-flowing cells.

Application L2102
New frontiers in enzyme immobilisation: robust biocatalysts for a circular bio-based economy

An interesting paper where the authors mentions ”A further refinement, the rotating bed reactor developed by SpinChem... This technology combines the benefits of an STR and a packed bed and has been scaled up successfully to more than 100 litres scale.” Key learnings from the paper (1) The advantages and limitations of immobilised enzymes in industrial applications (2) The different technical and regulatory requirements using immobilised enzymes (3) The different enzyme immobilisation methods (4) The different reactor technologies for immobilised enzymes and biocatalysis in general (5) Recent advances in enzyme immobilisation for better production economy

Production of recombinant choline oxidase and its application in betaine production

The paper describes the author’s successful work to express and use recombinant choline oxidase. The gene for the choline oxidase was isolated from a Gram-positive soil bacterium, cloned into an expression vector, inserted into and overexpressed in a Rosetta expression system. The isolated recombinant choline oxidase was subsequently immobilized onto Ni-Sepharose beads, which were loaded in a rotating bed reactor (RBR). With the immobilized choline oxidase in the RBR, reaction conditions such as pH and temperature were optimized and the enzymatic activity measured for the reaction of choline to glycine betaine via betaine aldehyde.

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