Application L2112

Production of hydroxytyrosol rich extract from Olea europaea leaf with enhanced biological activity using immobilized enzyme reactors

The paper describes work to valorize olive leaves, the major by-product in olive oil production.

Aqueous olive leaf extract is rich in oleuropein that was enzymatically hydrolyzed by beta-glucosidase to hydroxytyrosol and other products. Compared to the non-modified extract, the modified one demonstrated 20% higher antioxidant activity, seven-fold higher antibacterial activity, and enhanced cytotoxicity against leiomyosarcoma cells. The beta-glucoside was immobilized in chitosan-coated magnetic beads and used in both stirred tank reactor (STR) and rotating bed reactor (RBR).

The immobilized enzyme showed good stability over time and the activity was good in both STR and RBR settings.

However, the enzyme beads were damaged mechanically in the STR and could not be re-used. The RBR solved the problem of bead attrition allowing the immobilized enzyme to be re-used and thus giving superior total turnover number to the STR.

Details

Chatzikonstantinou AV, Giannakopoulou Α, Spyrou S, Simos YV, Kontogianni VG, Peschos D, Katapodis P, Polydera AC, Stamatis H. Production of hydroxytyrosol rich extract from Olea europaea leaf with enhanced biological activity using immobilized enzyme reactors. Environ Sci Pollut Res Int. 2021 Oct 22. doi: 10.1007/s11356-021-17081-6. Epub ahead of print. PMID: 34676481.

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