Application L2110

Process design of a continuous biotransformation with in situ product removal by cloud point extraction

In biotransformations, obstacles commonly encountered are product inhibition, product toxicity, and reaction equilibria that prevents complete conversion. Enzyme engineering has made tremendous progress in alleviating these problems. The concept of in situ product removal (ISPR) may still be an attractive alternative or complement.

The authors have demonstrated concurrent enzymatic reaction and ISPR, referred to as 'extractive biocatalysis'. For the ISPR, the authors evaluated the use of aqueous micellar two-phase systems (ATPMS) as an extraction medium. For the model reaction, Penicillin G hydrolysis by CalB lipase, the demonstrated process was thus a continuous, heterogeneous extractive biocatalysis with cloud point extraction.

An RBR was used during the process development work to determine the Michaelis-Menten kinetics of the CalB immobilized in gel coatings on column packing material.

Also, the particles were easily re-used in stability experiments.

Details

TITLE: Process design of a continuous biotransformation with in situ product removal by cloud point extraction Authors: Fellechner, Oliver; Smirnova, Irina Publication date: May 2021 DOI: 10.1002/cjce.23967

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